Coding

Part:BBa_K2324004:Design

Designed by: Rahan Nazeer   Group: iGEM17_Exeter   (2017-10-12)


FimH+SynMT


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 879
    Illegal AgeI site found at 306
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimisation for expression in E. coli . Research was required to identify the ideal placement of the foreign protein domain.


Source

The FimH sequence is a codon optimised version of the registry entry BBa_K1850001. The SynMT sequence is from *insert reference*.

References